Macherey-Nagel’s NucleoSpin 96 Tissue kit is designed for rapid purification of high molecular weight genomic DNA from tissue samples, such as mouse and rat tails, organ tissue, and animal or bacterial cells. The purified DNA can be used directly for a variety of downstream applications, including PCR, Southern blotting, genotyping, NGS, or any kind of enzymatic reaction.
The NucleoSpin 96 Tissue kit is convenient for high-throughput DNA extractions, but the many pipetting steps required in the manual processing of samples are tiring for users and introduce the opportunity for pipetting errors at each step. Performing the assay on the Assist Plus pipetting robot eliminates the possibility of pipetting errors and provides straightforward semi-automated sample extraction, eliminating the possibility of pipetting errors and reducing the strain of repetitive pipetting.
This application note from Integra Biosciences provides a protocol for automating Macherey-Nagel’s NucleoSpin 96 Tissue extraction and purification kit using the Assist Plus pipetting robot to eliminate pipetting errors and reduce the strain of repetitive pipetting.
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