The use of silica membrane-based DNA purification kits is a convenient way to prepare high-quality, transfection-grade plasmid DNA samples for cloning, sequencing, and restriction analysis or more sensitive applications, such as transfection of standard cell lines. In the latter case, transfection efficiency is negatively impacted by endotoxins, so it is important that levels are low.
Macherey-Nagel has developed a novel technology to reduce endotoxin content. Its NucleoSpin 96 Plasmid Transfection-Grade kit is designed for high throughput purification of high-copy plasmid DNA from E. coli in a 96-well plate format. The miniprep protocol is based on alkaline lysis and is optimized for the purification of plasmid DNA from 1-5 ml of bacterial culture. However, it is a time-consuming step in genetic analyses.
This application note from Integra Biosciences describes the use of the Assist Plus pipetting robot and the Viaflo 12-channel electronic pipette to automate plasmid DNA isolation with the Macherey-Nagel NucleoSpin 96 Plasmid Transfection-Grade kit and NucleoVac 96 Vacuum Manifold to offer more hands-free time and increase reproducibility.
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