This application note describes how to set up a 384-well qRT-PCR assay by hand, using a VIAFLO 16 channel 125 μl electronic pipette to distribute master mixes of two genes of interest into a 384 well plate.
All 64 samples are loaded directly from 1.5 ml microcentrifuge tubes into the 384 well plate in triplicate, using a VOYAGER 8 channel 12.5 μl adjustable tip spacing pipette. The whole process takes less than 10 minutes in real time.
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