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Tackling the Challenges of Serum Proteomics
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"Tackling the Challenges of Serum Proteomics"

Proteomic workflows that support serum proteomics can be especially challenging for two reasons

Proteomic workflows that support serum proteomics can be especially challenging for two reasons: 1) the presence of highly abundant proteins and 2) a particularly proteolytic resistant sample type due the large concentration of antibodies present. Many proteomic enrichment strategies employ the use of immuno-affinity depletion to remove one or more high-abundance proteins. Some common limitations of immuno-affinity, however, are high costs, regeneration requirements that may result in a diminished and inconsistent performance, as well as a required marriage of species to antibody. Because of these limitations, researchers need ways to enrich differently. 

This white paper outlines the serum proteome bias characteristics of AlbuVoid & NuGel Protein A, alone or in serial combination, using LC-MS reporting metrics. Products and digest conditions produce different proteome qualitative and quantitative windows of observation.


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