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Much of the versatility of the CRISPR system comes from the CRISPR-associated protein, or Cas protein, the molecular scissors that recognize and cut specific pieces of DNA. Since the initial discovery of what would become known as CRISPR, six major types and more than 22 subtypes of Cas proteins have been identified. Cas12 is a more recent addition to the CRISPR pantheon, gathering particular interest for its potential use in diagnostics.

This white paper from ERS Genomics describes the major differences between Cas9 and Cas12 CRISPR systems and discusses specificity, reliability, and patent considerations for choosing a system for commercial, research, and diagnostics applications.

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