Recombinant spike proteins are a critical resource for functional and structural SARS-CoV-2 studies.
This application note from DNA Script demonstrates the use of primers produced with novel enzymatic DNA synthesis technology in a site-directed mutagenesis strategy to produce optimized and variant SARS-CoV-2 spike protein expression plasmids, showing the primers to support efficient multi-site mutagenesis and Sanger sequencing confirmation of mutations and cloned inserts.
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