Real-time PCR quantification, also called quantitative PCR (qPCR) compares cycle quantification values of unknown samples to that of standards containing known amounts of nucleic acids. Contrary to qPCR, digital PCR (dPCR) enables absolute quantification without the need for a reference standard. Digital PCR systems claim to provide higher precision and reproducibility than qPCR.
This application note compares the precision and reproducibility of the Crystal Digital PCR (cdPCR) technology using the Naica System to that of qPCR technology.
The study compared the quantification variability of 23 qPCR and cdPCR technical replicates from a single unique PCR master mix spiked with a final concentration of 175 cp/µl of human genomic DNA.
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