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The chromatin conformation capture method Hi-C has been widely adopted to assess the frequency of interaction between distant genomic locations on a genome-wide scale. The resolution at which the interaction point can be mapped is reliant on two factors: the distance between fragmentation sites and uniformity of fragmentation length.

While restriction enzymes (RE) are widely used for chromatin fragmentation in Hi-C protocols, they are not uniformly distributed across the genome, thereby generating fragments of highly variable length.

Dovetail Genomics has addressed this shortcoming by using micrococcal nuclease (MNase) in its Micro-C Proximity Ligation Assay. The resulting highly uniform, short fragments enable nucleosome-level resolution of chromatin contacts.

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