The Hi-C chromatin capture method is usually performed using restriction enzymes (RE) to digest chromatin prior to a proximity-based ligation step, but there are notable drawbacks to this approach, including the fact that RE-based Hi-C is blind to approximately 20 percent of the mappable human genome due to low RE site density.
This application note introduces Omni-C, a sequence-independent, endonuclease-based proximity-ligation assay that addresses the limitations of RE-based Hi-C approaches.
Omni-C libraries contain proximity-ligation characteristics with the advantage of uniform genome-wide coverage. This expanded data type enables not only chromatin conformation studies, but also applications relying on coverage uniformity, such as SNP calling, chromosome phasing, and structural variant detection.
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