This technical note provides guidelines for performing automated chromogenic co-detection of RNA and protein on the Leica BOND RX System.
The Integrated Co-Detection Workflow (ICW) combines ACD’s chromogenic Red ISH detection assays using either the RNAscope 2.5 LS Reagent Kit RED (Cat. No. 322100) or the BaseScope LS Reagent Kit (Cat. No. 323600) with fully automated DAB or semi-automated Green immunohistochemistry (IHC).
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