Translational and exploratory research require high-plex analyte profiling to examine cellular changes across biological systems. The recent launch of Digital Spatial Profiling with Next Generation Sequencing readout technology (DSP-NGS) enables a vast increase in the number of targets that can be profiled with DSP.
This whitepaper introduces the GeoMx Human Whole Transcriptome Atlas (WTA) for DSP, provides general guidelines for region selection to optimize performance, and compares the assay to other technologies to bridge research from lower plex or bulk technologies. This work utilizes the Human WTA, but the guidance can be extended to other GeoMx RNA assays such as the Mouse WTA.
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