The reprogramming of human induced pluripotent stem cells (iPSCs) has revolutionized regenerative medicine, but gaining an accurate and quantitative understanding of signaling networks that regulate stem cell differentiation is crucial and remains a daunting challenge. The complexity of signaling networks stems from the many proteins and phosphorylated protein isoforms involved, and researchers are often challenged with limited material for analysis.
Traditional methods like western blot lack throughput, multiplexing ability, detection sensitivity, and reproducible quantification needed to gain a complete understanding of stem cell biology. Simple Western is an advanced capillary immunoblotting platform that addresses the limitations of current methods for protein expression and cell signaling pathway analysis in stem cells for regenerative medicine.
This application note from Bio-Techne shows how the automated western blot analysis of Simple Western can be used to monitor iPSC differentiation into the primary germ layers of human embryos, applying high-sensitivity multiplex assays to reveal and quantify changes in the expression of factors that regulate stem cell differentiation.
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