Single-cell RNA sequencing (scRNA-seq) of minimally invasive liquid biopsies, such as blood, bone marrow, urine, and cerebrospinal fluid enables researchers to gain valuable biological insights into tissue-resident diseases.
Sites where these samples are collected often do not have the specialized equipment or trained personnel required by many scRNA-seq technologies. Freezing and shipping single-cell samples to a centralized lab setting for processing into sequencing libraries allows for greater flexibility with multi-site and time course collections.
Cryopreservation, a common storage method, can be irreproducible and cause poor cell viability and cell stress due to ice crystal formation during freezing, leading to poor data quality. The Hive CLX scRNA-seq Solution integrates sample storage and single-cell profiling into a single workflow. After cells are gently loaded into the Hive collector, the Cell Preservation Solution locks the molecular profile at the time and site of collection. The quantity, quality, and biological complexity of cells stored in the Hive device are comparable to freshly processed cells, so these important samples can be stored or shipped without compromise.
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