As next generation sequencing (NGS) experiments generate vast amounts of data, selecting the right analytical approach and bioinformatics tools is crucial for extracting useful information while avoiding misinterpretation or bias.
This guide from Azenta Life Sciences provides a brief introductory tutorial to whole genome sequencing (WGS) and RNA-Seq bioinformatics, focusing on short-read Illumina sequencing data, which is commonly used for both WGS and RNA-Seq experiments. The guide also provides an overview of the hardware, software, and skills required for WGS analysis, emphasizing the importance of a Linux environment, adequate RAM and storage, and familiarity with command-line tools and scripting languages.
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