Generation of robust NGS results relies largely on the quality of the library, making analysis of the libraries a crucial step in the sequencing workflow. The library preparation workflow, while critical, is also time-consuming, labor-intensive, and expensive. Implementing NGS sample quality control within library preparation protocols enables laboratories to conserve valuable time and resources.
The Agilent automated electrophoresis solutions offer fast and reliable quality control of next-generation sequencing (NGS) libraries. The Bioanalyzer, TapeStation, and Fragment Analyzer systems with dedicated high-sensitivity assays allow unambiguous detection of daisy chains and accurate sizing of the target library.
This application note from Agilent provides recommendations for quality control assays to employ to reliably visualize daisy chain products in NGS libraries and emphasizes the consistency between instruments and reproducibility of analysis confirmed by results on a set of two-fold serial dilutions.
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