Scientists quantify protein content in samples using both direct and indirect measurements.
An example of a direct measurement is the Protein A280 application, which calculates protein concentration based on the sample absorbance at 280 nm and the protein- and wavelength-specific extinction coefficient. Colorimetric assays are an example of an indirect measurement.
This compendium provides technical documents, application notes, and protocols for measuring proteins using the Thermo Scientific NanoDrop One/OneC Microvolume UV-Vis Spectrophotometer. The compendium also covers the instrument software which is hardcoded with applications to measure the product of the BCA, Bradford, Lowry, and Pierce 660 nm reactions.
Offered Free by: Thermo Fisher Scientific
See All Resources from: Thermo Fisher Scientific