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Cell culture is a technique in which cells are grown outside a living organism under well-defined, controlled conditions.

It is important for basic biological research and a wide range of clinical studies. Mass culture of cells is indispensable for producing viral vaccines, enzymes, synthetic hormones, anticancer agents, recombinant proteins, and antibodies, but also for studying the physiology and biochemistry of cells. Nowadays, cell culture is also used in agriculture for producing alternative foods. Preserving cell viability, avoidance of contamination, high experimental reproducibility, and accuracy are vital for manipulations carried out on cells during media changes, transfers, seeding, and passaging.

This application note from Integra Biosciences provides a step-by-step protocol for using Viaflo handheld electronic pipettes for reproducible cell washing, transfer, and seeding steps in the culture of mouse embryonic stem cells.

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