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"Pre- and Post-Lyophilization Performance of RapiDxFire qPCR 5X Master Mix GF in Conjunction with a BioAcumen ASFV Assay"

Formulated with diagnostic kit developers in mind, RapiDxFire qPCR 5X Master Mix GF (GF denotes glycerol-free) from LGC Biosearch Technologies can be combined with gene‑specific primers and hydrolysis probes for immediate use in high-throughput laboratory‑developed test workflows or further processed for lyophilization for use in point-of-care point-of-care devices.

Provided at a 5X concentration without passive reference dye, this flexible master mix allows more reaction volume for sample and can detect up to 5 targets simultaneously. The RapiDxFire qPCR 5X Master Mix GF, manufactured in an ISO 13485-certified facility, is available in a range of pack sizes, from small sizes for developmental work to large-scale production batches, ideal for diagnostic test development workflows.

This application note from LGC Biosearch Technologies demonstrates the performance of RapiDxFire qPCR 5X Master Mix GF pre- and post‑lyophilization using an African Swine Fever Virus ASFV detection assay designed by BioAcumen Global.

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