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PCR Reaction Setup and AMPure XP Application
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"PCR Reaction Setup and AMPure XP Application"

This technical note outlines the he Biomek 4000 Laboratory Automation Workstation, which has been used to demonstrate highly reproducible real-time amplification (CV<2%) after automated reaction setup.

In addition, the Agencourt AMPure XP reagent system has been automated on the Biomek 4000 Workstation to provide rapid amplicon purification (two 96-well plates in less than 90 minutes) with yield and purity equivalent to manual purification. Both procedures were demonstrated to be free of cross-contamination.

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