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Multi-Organism Transcriptomics with Zymo-Seq RiboFree Total RNA Library Kit
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"Multi-Organism Transcriptomics with Zymo-Seq RiboFree Total RNA Library Kit"

Generating accurate and informative RNA sequencing data for transcriptomics research can be difficult due to the exceedingly high proportion of ribosomal RNA (rRNA). Although several rRNA removal strategies have been developed to aid in RNA-seq library preparation, probe-free rRNA depletion has been reported as more versatile compared to other methods, especially for its potential universal compatibility with different classes of organisms.

The Zymo-Seq RiboFree Total RNA Library Kit has been developed and optimized based on this novel rRNA depletion strategy, which has been quickly adopted by researchers for transcriptomic studies in animals, plants, and microorganisms.

This application note describes a whole-transcriptome analysis on several model organisms using the RiboFree kit to further validate its cross-species compatibility.


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