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Measuring Dead Volume in INTEGRA's Multichannel Reagent Reservoirs
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"Measuring Dead Volume in INTEGRA's Multichannel Reagent Reservoirs"

Any loss of reagent due to a high dead volume is unacceptable, yet this is a reality that many researchers face on a daily basis when using a multichannel pipette to transfer a reagent from a reservoir into a multiwell plate.

Most reagent reservoirs feature a V-shaped bottom to minimize dead volumes. However, as the liquid level lowers, the reagent starts to pool unevenly. As soon as a single pipette tip aspirates air, liquid transfer has to stop and the reagent remaining cannot be used for the defined application. This is known as the dead volume.

INTEGRA developed a novel 25 ml divided reservoir to overcome this issue, which combines two innovative features: the SureFlo anti-sealing array and a unique surface treatment that spreads liquid evenly to offer even lower dead volumes. INTEGRA’s two-compartment reservoir enables the pipette tips to sit on the bottom and prevents liquids from pooling, offering the lowest dead volume on the market.

This technical note provides experimental data to demonstrate this, and guidance to support users in setting up their experiments.


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