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"KAPA RNA HyperPrep Workflow: Recommendations and Expectations for RNA-Sequencing Using Degraded Inputs"
"KAPA RNA HyperPrep Workflow: Recommendations and Expectations for RNA-Sequencing Using Degraded Inputs"
The quality of RNA extracted from biological specimens is highly variable and yields are often low, thus impacting the ability to generate high-quality sequencing libraries.
In this study, the effects of RNA type and quality on RNA-seq library construction are assessed and expectations regarding sequencing data quality are addressed. Included are recommendations for informative quality control measures and input-specific modifications that may improve performance.
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