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"Improving and Simplifying the Design of Knock-In Experiments Using the TrueDesign Genome Editor"

Genome editing technologies have given investigators the power to unlock a variety of new applications and experimental approaches. While gene knockouts have been achievable for several years, successful gene knock-ins have remained elusive due to the low efficiency of homology-directed repair (HDR).

Successful knock-in experiments depend on the design of the donor DNA, the effectiveness of the single guide RNA (sgRNA) and Cas9 ribonucleoprotein (RNP) complex, and the efficiency of delivering materials into the target cells.

This application note from Thermo Fisher Scientific describes two use cases for the Invitrogen TrueDesign Genome Editor, a free online tool for designing and ordering materials for knock-in CRISPR–based editing: introduction of an SNP change in the LRRK2 gene in induced pluripotent stem cells and tagging of beta-actin with GFP.

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