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Single-cell RNA sequencing enables simple and robust access to the transcriptomes of thousands of single cells -- giving insight into tissues at single-cell resolution. This offers vital information about heterogeneity and cell-specific dynamics, which is key to understanding many diseases, immunity, development, and more.

The Dolomite Bio Nadia Instrument uses automation and flexibility to generate reproducible, high-quality single-cell data at high throughput to assay tens of thousands of cells whilst maintaining a low cost per cell. This application note describes results obtained from the encapsulation of single cells with barcoded mRNA capture beads for single-cell RNA sequencing using the Drop-seq protocol on the Nadia Instrument.

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