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"HD-Seq: A Novel Method for High-Accuracy Sequencing"

Next-generation sequencing (NGS) has enabled rapid progress in cancer diagnostics. However, challenges remain around the detection of somatic variants at allele frequencies similar to or below the error rate of on-market DNA sequencers (typically a ~10-3) when using standard library preparation methodologies.

This poster from Singular Genomics introduces HD-Seq, a novel library preparation and sequencing method that relies on linking the original paired strands in a dsDNA fragment and that allows for efficient and high-accuracy sequencing, achieving error rates in the range of ~10-5-10-6.

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