Bulk RNA-sequencing is commonly used to analyze RNA expression from a population of cells in suspension.
However, this method hasn’t traditionally allowed for protein detection in the same sample. Our workflow, developed in collaboration with Illumina, stains cells with TotalSeq™ oligo-conjugated antibodies prior to bulk RNA sequencing, enabling the simultaneous profiling of cell surface proteins.
Download our application note to learn more about the utility of bulk epitope and nucleic acid sequencing (BEN-seq) and see how we used the technique to detect 280 proteins and measure the Th1 response on both unstimulated and stimulated cells.
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