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Encapsulation of Cells in Agarose on the Nadia Innovate and Nadia Instrument
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"Encapsulation of Cells in Agarose on the Nadia Innovate and Nadia Instrument"

Conventional plate-based cell culture methods attempt to simulate a cell-growth environment in a two-dimensional plane. In contrast, encapsulating biological material within hydrogel droplets allows cells to be grown within three-dimensional scaffolds, more closely mimicking their native physiological environment.

The availability of microfluidic devices, such as the Nadia Instrument, allow the co-encapsulation of two distinct cell types in agarose for high-throughput analysis of single cell-cell or cell-pathogen interactions over biologically relevant timeframes.

This application note describes the encapsulation and cultivation of mammalian cells in agarose beads using the Nadia Innovate, illustrating the instrument’s potential in the field of single-cell analysis in hydrogel scaffolds.

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