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Efficient Two-Way Sample Pooling Automated with the Assist Plus
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"Efficient Two-Way Sample Pooling Automated with the Assist Plus"

In diagnostics, sample pooling is an efficient method to increase sample testing while reducing reagent use.

Traditional pool construction requires combining equal portions of specimens into one vessel. Positive sample pools trigger retesting of each sample in that pool. In two-way pooling, the specimens are pooled in a grid pattern. Samples are first pooled horizontally, and then vertically. In this way, each sample is tested in 2 separate pools. Positive samples will yield a positive result in 2 pools, allowing direct identification of the individual positive sample, minimizing the need for confirmatory testing.

This application note from Integra Biosciences provides a procedure for automating two-way sample pooling in diagnostic assays with the Assist Plus pipetting robot, increasing sample testing while reducing reagent use and sources of error.


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