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Drop-off Crystal Digital PCR for NRAS, KRAS, & EGFR Mutations
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"Drop-off Crystal Digital PCR for NRAS, KRAS, & EGFR Mutations"

A major advantage of a drop-off digital PCR is the single-assay detection of multiple proximal genetic lesions (including deletions, insertions, and nucleotide substitutions) within a short genomic interval.

This application note describes a study that used Crystal Digital PCR to design and validate three internally controlled drop-off assays for the detection of seven KRAS mutations, four NRAS mutations, and a range of EGFR exon 19 deletions/insertions commonly monitored in clinical practice.

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