This application note describes a fully automated protocol for generating quantified mRNA libraries -- including QC and library normalization -- using the DreamPrep NGS workstation and the Universal Plus mRNA-Seq library preparation kit.
The protocol enables reproducible mRNA library preparation with minimal user intervention, quantifying and normalizing the libraries without sample loss. This set-up eliminates the need for additional library quantification steps, saving time, resources, and library material.
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