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Development and Validation of a Quantitative Synthetic Molecular Standard for Monkeypox Virus

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"Development and Validation of a Quantitative Synthetic Molecular Standard for Monkeypox Virus"

Human monkeypox virus (hMPXV) is a zoonotic viral disease endemic to central and western Africa, causing a smallpox-like disease in humans. Rapid and accurate detection of hMPXV during early stages of infection is essential for containment and proper treatment.

While culture-based approaches can be used to detect hMPXV, they are time-consuming and labor-intensive, making PCR-based methods a more attractive alternative for rapid and highly sensitive screening.

To address the need for high-quality reference materials in PCR assay development and validation, ATCC designed and developed a quantitative synthetic molecular standard for hMPXV, containing biomarkers for Clade I (Congo Basin) and Clade II (West African). The standard was authenticated via next-generation sequencing and quantified using droplet digital PCR via published quantitative PCR assays. The synthetic DNA construct proved compatible with all assays tested, demonstrating its potential as a control for assay development, verification, and validation.

This white paper from ATCC discusses the development and validation of this standard.

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