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Detection of Hypermethylated Circulating Tumor DNA by Crystal Digital PCR
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"Detection of Hypermethylated Circulating Tumor DNA by Crystal Digital PCR"

In colorectal cancer, hypermethylation of WNT inhibitory factor 1 (WIF1) and neuropeptide T (NPY) was found in 80 percent and 44.7 percent of metastatic and stage II/III patients, respectively.

This application note describes a study that sought to evaluate whether hypermethylated WIF1 and NPY can be used as a universal colorectal cancer marker and a surrogate to tumor sequence-specific mutations.

The method, which combined bisulfite conversion of unmethylated cytosine to uracil, followed by 3-color Crystal Digital PCR, enabled the reliable detection of down to 25 and 5 copies of hypermethylated WIF1 and NPY DNA, respectively, per 25 µl reaction.


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