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Custom Library Adaptors Via Enzymatic DNA Synthesis Improve Precision of Real-Time Nanopore Sequencing
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"Custom Library Adaptors Via Enzymatic DNA Synthesis Improve Precision of Real-Time Nanopore Sequencing"

This on-demand GenomeWebinar, sponsored by DNA Script, discussed how benchtop enzymatic DNA synthesis enables the rapid creation of oligonucleotides that can be combined with real-time nanopore DNA sequencing to accelerate iterative design and testing cycles in synthetic biology.

Tim Mercer of the Australian Institute of Bioengineering and Nanotechnology shared how his team uses enzymatic DNA synthesis to build novel, custom library adaptors, termed CAPTORS, that contain functional sequence elements that can measure the qualitative and quantitative accuracy of a DNA sequencing library. Dr. Mercer demonstrated how CAPTORS are used to prepare libraries from patient samples, with the functional information retrieved during nanopore sequencing.

CAPTORS can also analyze quantitative accuracy, improving measurements of gene expression in mRNA samples, and enabling best-in-class normalization between samples across large patient cohorts, longitudinal patient timelines, and point-of-care testing. These benefits can also be employed to improve clinical diagnosis, with CAPTORS able to sufficiently mitigate nanopore sequencing errors to improve the diagnosis of pathogenic BRCA1/2 variants in breast cancer.

Together, this demonstrates how the enzymatic synthesis of CAPTORs can affix information within patient samples during library preparation that is later retrieved by sequencing. The information encoded within CAPTORS can act as internal ‘software’ that interfaces with external bioinformatics software for responsive analysis and can integrate patient samples into a large decentralized global network or a clinical IT infrastructure.


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