A challenge of RNA sequencing is to efficiently eliminate abundant or uninformative sequences, such as ribosomal RNA (rRNA), to shift discovery to the lower expressing and biologically interesting transcripts. CRISPRclean Stranded Total RNA Prep with rRNA Depletion (Human, Mouse, Rat) uses CRISPR-based depletion with stranded RNA library preparation to specifically remove human, mouse, and rat rRNA sequences from adapter-ligated cDNA libraries.
CRISPRclean Stranded Total RNA Prep with rRNA Depletion reassigns sequencing reads from abundant molecules to higher value and lower expressing transcripts in an unbiased manner without disturbing the relative transcript abundance or complexity of the library.
This white paper from Jumpcode Genomics describes an experiment that used this CRISPR-powered rRNA depletion strategy to deplete mammalian rRNA in tissue samples, improving sensitivity for the detection of lower expressing transcripts.
Offered Free by: Jumpcode Genomics
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