Glen Research provides several unique, base-modified 2’-deoxynucleoside monomers for site-specific modification of oligonucleotides. These synthons are known as convertible nucleosides, and the process for use in oligo modification is referred to as “post-synthetic substitution.” Since their introduction, convertible nucleosides have proven useful in physical-chemical studies and studies of DNA replication, error, and mutation potential.
This note provides an introductory overview of these molecules and a brief update of the key chemistry milestones since their introduction. This note also reviews a recent research application using convertible dG that takes advantage of the site-specific preparation of a DNA lesion to investigate translesion synthesis (TLS) properties of mammalian cell polymerases and the accuracy of Y-family DNA polymerases ĸ (kappa) and ι (iota).
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