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The human gut microbiome plays an important role in human health and various diseases, including many with no existing obvious correlations with the gut microbiome. Interest in using high-throughput DNA sequencing techniques to study the human gut microbiome has grown exponentially across many sectors, from academia to industry to the government.

This white paper from Zymo Research uses real-world examples to discuss important considerations for building a shotgun metagenomics workflow for microbiome profiling, including microbiome quality control, sample collection and preservation, DNA extraction, library preparation, sequencing, and analysis.

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