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"Comparison of Evercode WT v2 and Chromium Next GEM Single Cell 3' Kit v3.1 in Mouse Brain Nuclei"

The brain is one of the most complex and heterogeneous organs in terms of its cell types. This technical note compares two single-cell RNA sequencing technologies, the 10x Genomics Chromium Next GEM Single Cell 3’ Kit v3.1 and the Parse Biosciences Evercode WT v2, using mouse brain nuclei as a sample type.

The study evaluates gene detection, cell type recovery, and differential gene expression detection for both technologies. The Evercode WT v2 consistently outperformed the Chromium Next GEM Single Cell 3’ Kit v3.1 in gene detection at all sequencing depths, recovered similar proportions of cell types, and detected substantially higher numbers of differentially expressed genes across all cell types. The results suggest that Evercode WT v2 yields higher sensitivity and more information per cell, making it a valuable tool for single-cell RNA-seq studies in neurobiology.

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