Comprehensive spatial analysis is ideally accomplished by combining multiplexing technologies that allow simultaneous visualization of multiple target genes within the same sample with image analysis tools that can provide quantitative, single-cell level data to accurately interpret gene expression within the context of the tissue.
This application note from ACD demonstrates how the Halo image analysis platform can be employed with the RNAscope HiPlex v2 assay to quantitatively assess differential expression of 12 RNA targets, quantify six distinct cell phenotypes based on gene expression, and analyze spatial relationships between cell phenotypes within the Tumor Microenvironment.
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