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Capturing Tumor Heterogeneity with Rapid, Deep, Spatial Phenotyping
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"Capturing Tumor Heterogeneity with Rapid, Deep, Spatial Phenotyping"

Spatial phenotyping is the simultaneous visualization and quantification of dozens of biomarkers in situ using a single sample while maintaining cellular and sub-cellular resolution. Analyzing multiple proteins in conjunction within a single tissue section can reveal unexpected patterns and associations that are not evident with other methodologies.

Spatial phenotyping also allows the investigation of nuanced behaviors a cell exhibits in its native cellular microenvironment. Identification of distinct spatial cellular behaviors is a valuable tool in the identification of potential therapeutic targets and is predictive of the morbidity and mortality associated with a given tumor.

This application note from Akoya Biosciences introduces an ultra-high-plex single-cell spatial phenotyping study of human FFPE head-and-neck tumor tissue aimed at unveiling orchestrated and complex tumor heterogeneity, demonstrating the PhenoCycler-Fusion technology that can provide an integrated view of the pathways that regulate tumor progression.


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