Improving Plex, Resolution, and Throughput in Spatial Biology
A compounding of requirements in spatial phenotyping for truly characterizing the complexity of a tissue microenvironment has culminated in an iron triangle, a concept by which resolution, plex, and throughput become extremely challenging to simultaneously improve, and therefore, a tradeoff must be made. For example, single-cell technologies, such as flow cytometry and single-cell RNA-seq, enable the detection of numerous parameters but lack spatial dimension. Emerging spatial technologies have alleviated this concern but rely on spot-based or region-of-interest-based methods, which lack resolution. On the other hand, standard imaging methods, like immunofluorescence and immunohistochemistry, enable single-cell resolution and provide spatial context but are limited to measuring just a few parameters simultaneously.
In this on-demand webinar from Akoya Biosciences, speakers define spatial phenotyping, discuss how new technologies are improving resolution, plex, and throughput, and provide examples of work scientists are performing to advance the field of spatial biology.
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