Automating qPCR master mix preparation is often considered extremely tedious, due to the need to constantly change program set-up based on varying master mix volumes and sample numbers. However, the D-One single-channel pipetting module for the Assist Plus pipetting robot simplifies the programming of automated qPCR master mix preparation protocols down to a single step.
The D-One individually reaches tubes of different qPCR master mix components, while the Vialab program takes care of all calculations and provides automatic tip selection.
This application note from Integra verifies a SYBR Green qPCR protocol prepared directly in a divided reservoir using the D-One single-channel pipetting module for the Assist Plus pipetting robot, allowing master mixes to be pipetted into any tube or reservoir, enabling large-scale qPCR master mix preparation, with subsequent distribution to plates with multichannel pipettes to speed up the process.
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