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"Automated Preparation and LC-MS/MS Analysis of Methylmalonic Acid in Serum Using the Andrew+ Pipetting Robot and Xevo TQ-S Micro"
The analysis of methylmalonic acid (MMA) can be used to determine the status of vitamin B12 serum levels. MMA is a preferable indicator to vitamin B12 due to the possibility of normal or high vitamin B12 serum levels even in deficient individuals. The use of liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis for research into this biomarker is ideal for high throughput applications.
This application note from Waters Corporation demonstrates the automated extraction of MMA from serum employing the Ostro Plate for protein precipitation and phospholipid removal on the Andrew+ Pipetting Robot. The development of the MMA Andrew+ protocol takes advantage of the existing MMA workflow and LC-MS analytical method which allows for the adoption of high throughput sample preparation.
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