During analysis of drugs of abuse in urine, the drug metabolites (e.g. morphine) can be present as the glucuronide form. In these cases, hydrolysis using a β-glucuronidase enzyme is performed before liquid chromatography-mass spectrometry (LC-MS) analysis of the samples to ensure that the free form of the drug can be analyzed in the samples under investigation.
Subsequently, the sample requires a cleanup before injection into the LC-MS instrument. Solid-phase extraction remains the most convenient method for use in such sample cleanup.
This white paper from Millipore Sigma demonstrates the ability to perform cleanup of urine samples using HLB solid-phase extraction for the analysis of opioids via tandem mass spectrometry after cleanup with the Supel Swift HLB SPE 96-well plate.
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