CRISPR gene editing technology has the potential to transform research at an astonishing rate. The rate of success is dependent upon many factors, such as gRNA design, Cas9 efficiency, and delivery conditions.
This application note from Thermo Fisher Scientific demonstrates an end-to-end workflow, from T-cell isolation and activation to gene editing, that demonstrates over 90 percent functional knockout of the T-cell receptor in donor-derived primary T-cell populations.
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