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"A Tale of Two Platforms: Hive scRNA-seq Solution Recovers Complete Biology in Comparison to Droplet Method"

Many single-cell RNA sequencing (scRNA-seq) approaches exist; most notable are droplet and picowell-based technologies.

Droplet-based, microfluidic platforms isolate a single cell and barcoded transcript-capture bead within an oil droplet. In picowell-based platforms, both cell and bead are isolated within a well. As part of the Hive scRNA-seq Solution, the Hive device, in which sample capture occurs, contains thousands of picowells pre-loaded with capture beads. Single-cell suspensions are gently loaded into the HIVE device, either by gravity or low-speed centrifugation.

This application note from Revvity presents data showing how the Hive scRNA-seq Solution allows for better recovery of fragile cell types from storage for single-cell RNA sequencing as compared to droplet-based systems.

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