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A High-Throughput Workflow for Profiling Vaginal Microbiota
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"A High-Throughput Workflow for Profiling Vaginal Microbiota"

Research has expanded our understanding of how microbiota affect reproductive health (e.g., fertility, susceptibility to sexually transmitted infections, pregnancy) and embryonic health. Much of the groundwork for vaginal microbe identification was established using traditional techniques like complex culture preparation, specialized microscopy, and sequencing.

Analyzing vaginal microbes by traditional techniques often takes days or weeks, and these techniques have sensitivity limitations that newer molecular biology methods do not. To better understand the relationships between vaginal microbiota and female reproductive health, a robust workflow for microbial nucleic acid extraction and analysis that is easier, faster, and less expensive than traditional microbiology workflows is needed.

This application note from Thermo Fisher Scientific describes a semi-automatable, end-to-end workflow for profiling commensal and pathogenic vaginal microbiota — including gram-positive and gram-negative bacteria, yeasts, viruses, and protozoa — compatible with multiple storage and collection solutions.


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