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"A Guide to RNA-Seq"

This e-book from Azenta Life Sciences provides an in-depth guide to RNA-seq, a high-throughput sequencing technique that has revolutionized biological research by enabling quantitative measurement of transcription.

The guide covers various aspects of RNA-seq, including its advantages over other technologies, the types of information it can provide, and the various RNA-seq assays available. It is intended for scientists with a solid background in molecular biology and a beginner-to-intermediate understanding of next-generation sequencing (NGS).

The guide delves into the RNA-seq workflow, detailing each step from experimental design to data analysis. It also provides guidance on key decisions researchers must make at the start of an NGS project, such as the type of assay and the number of samples to analyze. Additionally, the guide includes a comprehensive comparison of RNA analysis technologies, highlighting the benefits of RNA-seq over alternatives like DNA microarrays and expressed sequence tag sequencing.

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